The Inhibition Effect of 940 nm Diode Laser on Some Microorganisms Associated With Gingivitis

Muhamed Ibrahem Hazeem, Muthenna Shaban Rajab, Hadeel Mizher Younis

Abstract


Background :Poor oral hygiene lead to gingival inflammation due to the accumulation of plaque and microorganisms. Scaling and polishing was considered as an effective treatment of gingivitis. Several systemic and local antimicrobials were found to enhance the mechanical treatment. However, these antimicrobial agents may lead to bacterial resistance and systemic side effects in addi- tion to limitation in the accessibility to the affected areas. The aim of this study is to explore the effect of diode laser (940nm) in combination to the traditional mechanical treatment.

Materials and methods: twenty patients with bilateral gingivitis were selected. For each patient, one side of the mouth was treated traditionally. The other side was treated with 2 W diode laser (940nm) after scaling and polishing. Smears were taken from deepest points of the gingival sulci of both sides. The collected bacterial samples were inoculated in brain-heart infusion and inoculated into blood agar plates and MacConkey agar plates for 24 hours aerobically and 24-48 hours anaerobically (using jar and CO2 gas pack) at 37oC. The collected data of bacterial identification were tabulated in excel tables. Means, standard deviations, ANOVA test and p value were calculated.

RESULTS there is highly significant decrease in the identification of aerobic bacteria after using diode laser. Anaerobic bacteria were less identified, but they were completely absent after diode laser treatment.
Conclusion: Diode laser (940nm) could be successfully used adjunctive to mechanical scaling and polishing to integrate the antimi- crobial action within the gingival sulci.


Keywords


Diode laser, Gingivitis, bacteria .

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References


Azadeh M, Kermanshahi RK, Sadat naghavi N, Ghalayani P, Salamat F. The profile of pathogenic bacteria isolated from dental plaque induced gingi- vitis. International Journal of Molecular and Clini- cal Microbiology 2011;1:36-39.

Marsh PD, Martin MV. Oral microbiology. Fifth edition. Elsevier Limited 2009.p120.

Sánchez MC, Liama-Palacios A, Blanc V. Struc- ture, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from subgingival microbiota. J Periodontal Res 2011;46:252-260.

Igic M, Kesic L, Lekovic V, Apostolovic M, Mi- halovic D, Kostadinovic L, M ilasin J. Chronic gingivitis: the prevalence of periodontopathogens

and therapy efficiency. Eur J ClinMicrobiol Infect

Dis 2012;31:1911-1915.

Hojo K, Nagaoka S, Oshima T, Maeda N. Bacte-

rial interactions in dental biofilm development. J

Dent Res 2009;88(11):982-990. 6. Atanasovska-Stojanovska A. Modern approach to

the identification and elimination of periodontal infection. Macedonian Journal of Medical Scienc- es 2012;5(2):215-221.

Theodoro LH, Silva SP, Pires JR, Garcia Soares- GH,Pontes AEF, Zuzu EP, Spolidó DMP, Correa de Toledo BE, Garcia VG. Clinical and microbio- logical effects of photodynamic therapy associated with nonsurgical periodontal treatment. A 6-monthfollow-up. Lasers Med Sci 2012;27:687-693. 8. Birang R, Yaghini J, Adibard M, Kiany S, Moham- madi Z, Birang E. The effect of diode laser (980 nm wavelength) and chlorhexidin gel in the treat- ment of chronic periodontitis. Journal of Lasers in

Medical Sciences 2011;2(4):131-138.

Walker CB. Selected antimicrobial agents: mech- anisms of action, side effects and drug interaction.

Periodontology 2000:12-28.

Quirynen M, Teughels W. van Steenberge D. Mi-

crobial shifts after subgingival debridement and formation of bacterial resistance when combined with local or systemic antimicrobials. Oral Dis 2003.9(Suppl 1):30-37.

Sennhenn-Kirchner S, Klaue S, Wolff N, Mergeryan H, Borg von Zepelin M, Jacobs HG. Decontamination of rough titanium surfaces with diode lasers: microbiological findings on in vivo grown biofilms. Clin Oral Implants Res 2007;18:126-132.

Luan XL, Qin YL, Bi LJ, Hu CY, Zhang ZG, Lin J, Zhou CN. Histological evaluation of the safe- ty of toluidine blue-mediated photosensitization to periodontal tissues in mice. Lasers Med Sci 2009.24(2):162-166.

Romeo U, Palaia G, Botti R, Leone V, Rocca JP, Polimeni A. Non-surgical periodontal therapy as- sisted by potassium-titanyl-phosphate laser: a pilot study. Lasers Med Sci 2010;25:891-899.

Aoki A, Saski KM, Watanabe H, Ishikawa I. La- sers in nonsurgical periodontal therapy. Periodon- tal 2004;36:59-97.

Liebana J, Castillo AM, Alvarez M. Periodontal diseases: microbiological consideration. Med Oral Cir Bucal 2004.9(Suppl):75-91.

Schneider M, Kirfel, Berthold M, Frentzen M, Kraus F, Braun A. The impact of antimicrobial photodynamic therapy in an artificial biofilm mod- el. Lasers Med Sci 2012;27:615-620.

Alves VTE, de Andrade AKP, Toaliar JT, Conde MC, Zezell DM, Cai S, Pannuti CM, De Micheli G. Clinical and microbiological evaluation of high intensity diode laser adjutant to non-surgical peri- odontal treatment: a 6-month clinical trial. Clin Oral Invest 2013;17:87-95.

Kamma JJ, Vasdekis VGS, Romanos GE. The ef- fect of diode laser (980 nm) treatment on the sur- vival rate of gingival fibroblast cell cultures. La- sers Surg Med 2011;28:445-450.

Mortez A, Schoop U, Goharkhay K, Schauer P, Doetbudak O, Wernisch J. Treatment of periodon- tal pockets with a diode laser. Lasers Surg Med 1998;22:302-311.

Nussbaum EL, Lilge L, Mazzualli T. Effects of 810 nm laser irradiation on in vitro growth of bac- teria: Comparison of continuous wave frequency modulated light. Lasers Surg Med 2002;31:343- 351.

Wilson M. Lethal photosensitization of oral bac- teria and its potential application in the photo dy- namic therapy of oral infections. PhotochemPho- tobiolSci 2004;3:412-418.

Mortez A, Gutknecht N, Doertbudak O, Go- harkhay K, Schoop U, Schauer P. Bacterial re- duction in periodontal pockets through irradiation with a diode laser: a pilot study. J Clin Laser Med Surg 1997;15:33-37.

Ryden H, Persson L, Perber H, Bergstorm J. Ef- fect of low level energy laser irradiation on gingi- val inflammation. Swed Dent J 1994;18:35-41.

Hassan LM, Gizar R, Majeed LJ. Effect of di- ode laser 805 nm on the viability of some types of gram negative and gram positive pathogenic bac- teria. Iraqi Journal of Science 2010;51(4):665-669.

Qadri T, Miranda L, Tuner J, Gustafsson A. The short-term effects of low-level lasers as adjunct therapy in the treatment of periodontal inflamma- tion. J ClinPeriodontol 2005;32:714-719.

Pirnat S, Lukac M, Ihan A. Study of direct bacte- ricidal effect of Nd:YAG and diode laser parame- ters used in endodontics on pigmented and nonpig- mented bacteria. Lasers Med Sci 2011;26:755-761.

Eduardo Cd, de Freitas PM, Esteves-Oliveira M,Aranha ACC, Ramalho KM, Simőes A, Bel- lo-Silva MS, Tunér J. Laser phototherapy in the treatment of periodontal disease. A review. Lasers Med Sci 2010;25:781-792.

Savafi SM, Kazemi B, Esmaeili M, Fallah A, Modarresi A, Mir M. Effects of low-level He- Ne laser irradiation on the gene expression of IL-1beta, TNF-alpha, IFN-gamma, TGP-beta, Bfgf, and PDGF in rats gingiva. Lasers Med Sci 2008;23:331-335.

Andersen R, Loebel N, Hammond D, Wilson M. Treatment of periodontal disease by photodisin- fection compared to scaling and root planning. J Clin Dent 2007;18:34-38.




DOI: http://dx.doi.org/10.26477/idj.v35i2.144

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