Effect of Autoclave on Bacterial Growth Isolated from Different Types of Denture Adhesives
Abstract
Background: Different types of denture adhesives like powder or paste have been used to enhance the retention and stability in denture wearers, especially in patients with resorbed ridges , these adhesives should be free from microbial contamination in order to use safely inside patient mouth and avoid infection , the purpose of this study is to investigate the bacterial contamination of different adhesives pastes and powders and the effect of autoclaving in reducing the bacterial growth .
Materials and Methods:Six types of commonly available denture adhesives (three powders and three pastes) were tested for bacterial contamination by using culture on suitable media that contained either brain-heart infusion (BHI) ,blood agar, nutrient agar,MSA agar and others were inoculated with 0.5 gm samples of each adhesive. plates were incubated at 37C˚ for 24 hours in pre and post sterilization by using the colony forming unit for estimation the bacterial growth.
Results : The results revealed that , In pre sterilization test all adhesive types (powders and pastes ) contained Gram positive and Gram negative bacteria ,the results also revealed that Dentofix (paste type 1) has the lowest bacterial growth in which 40% without bacterial growth , also the results reported that 25% of adhesives were contaminated by Staphylococcus species and 23 % of adhesives were contaminated by Neisseria species .Dentofix paste was highly significant difference regarding the mean of bacterial count at both dilutions 1/10 and 1/100 among other adhesives with the lowest contamination in comparison to other types of denture adhesives in presterilization step.while after using sterilization by autoclave generally sterilization induce high significant difference in each type of denture adhesive after exposure to autoclave for 15 min at 121 °C 15 pound/inch it decreases the number of bacterial growth in all types. The results showed that powders adhesives had high contamination rate than pastes and their killing rate after sterilization was lower than pastes. Fittydent (paste type 3) was highly affected by sterilization than other adhesives in first dilution 1/10 when killing rate was 78.9% . all types of tested adhesives showing that in second dilution 1/100 sterilization more effective at this dilution and sterilization induce 100% killing in Dentofix ( paste type 1) , Bonyplus ( paste type 2) and Fittydent (paste type 3) .
Conclusions: The result of this study concluded that the powders denture adhesive have high bacterial contamination rate than the pastes and using of sterilization by autoclave must be done to increase killing rate.
Keywords
Full Text:
PDFReferences
Elif Yegin, Yusuf Ziya Akpinar,TevfikYavuz, Muhammed Ali Aslan. Effect of different denture adhesives on retention of complete dentures: an in vivo study. Journal of adhesion science and technology .2017;31 (18) :2044-2052
Kuebker W: Denture problems: causes, diagnostic procedure and clinical treatment. Retention problems. J. Quintessence Int.1984;15: 131- 41.
Smith P and McCord J: What do patients expect from complete dentures? J. Dent. 2004; 32: 3-7.
Aghdaee N, Rostamkhani F and Ahmadi M: Complications of complete dentures made in Mashhad dental school. J. Mashhad dental school, 2007; 31: 1-3.
Al RH, Dahl JE, Morisbak E et al. Irritation and cytotoxic potential of denture adhesives. Gerodontology 2005; 22(3): 177–183.
Nikawa H, Makihira S. Research projects related to complete dentures published in 2008 by members ofthe Japan Prosthodontic Society. J Prosthodont Res 2009; 53: 103–106.
Pradies G, Sanz I, Evans O et al. Clinical study comparing the efficacy of two denture adhesives in complete denture patients. Int J Prosthodont 2009; 22: 361–367
Coats A: Usage of denture adhesives. J. Dent. 2000; 28: 137- 40.
Grasso J: Denture adhesives: changing attitudes. J. ADA. 1996; 127: 90- 6.
Wilson M, McCord J and Watts D: Denture adhesives- in vitro evaluation. J. Dent. Research. 1990; 969 -70.
Redford M, Dury T and Kingman A: Denture use and technical quality of dental Prosthesis among persons 18- 74: United States, 1988-1991. J. Dent. Res. 1996; 75: 714-25
Slaughter A, Katz R, and Grasso J: Professional attitudes toward denture adhesives: A Delphi Technique survey of academic prosthodontists. J. Prosthet. Dent. 1999; 82: 80-9.
Essam Adel Aziz, Azza Adel Aziz, Dina Essam Eldeen ,Ibrahim and Ali Eldeen, Mohammed Ahmed Comparative Study between Different Denture Adhesives in Improving Phonation in Complete Denture Wearers Journal of American Science 2010; 6(11):556-561
Grasso JE. Denture adhesives. Dent Clin N Am 2004; 48: 721–733.
Nations SP, Boyer PJ, Love LA et al. Denture cream: an unusual source of excess zinc, leading to hypocupremia and neurologic disease. Neurology 2008; 71: 639–643.
A study to evaluate the retentive ability of different denture adhesive materials: an in vitro study.J Indian Prosthodont Soc. 2010 ;10(3): 176–181.
Adisman IK. The use of denture adhesives as an aid to denture treatment. J Prosthet Dent 1989; 62: 711–715
Ruta Jadhav, Sounyala Rayannavar, Raghunath Patil, Vinuta Hiremath, Sumati A Hogade Effect of denture adhesive and its forms on microbial colonization in diabetic and nondiabetic complete denture wearers: An in vivo study Indian journal of health sciences and biomedical research Kleu 2017 ; 10(2) : 131-137
William D. Gates DDS, MS, Millicent Goldschmidt PhD and Donald Kramer DDS, MSMicrobial contamination in four commercially available denture adhesives J prosthetic dentistry 1994 ; 71 : 154-158
Sabrina Pavan, João Neudenir Arioli Filho, Paulo Henrique Dos Santos, Francisco de Assis Mollo Jr Effect of microwave treatments on dimensional accuracy of maxillary acrylic resin denture base Braz. Dent. J 2005 ;16 (2) .
Najdovski L, Dragas AZ, Kotnik V. The killing activity of microwaves on some non-sporogenic and sporogenic medically important bacterial strains. J Hosp Infect 1991;19:239-247.
Griffith D, Nacey J, Robinson R, Delahunt B. Microwave sterilization of polyethylene catheters for intermittent self-catheterization. Aust N Z J Surg 1993;63:203-4.
Rosaspina S, Anzanel D, Salvatorelli G. Microwave sterilization of enterobacteria. Microbios 1993;76:263-270.
Rohrer MD, Bulard RA. Microwave sterilization. J Am Dent Assoc 1985;110:194-198.
Webb BC, Thomas CJ, Harty DWS, Willcox MDP. Effectiveness of two methods of denture sterilization. J Oral Rehabil 1998;24:416-423
Eric J. Westergard, Laura M. Romito, Michael J. Kowolik, Charles John Palenik Controlling bacterial contamination of dental impression guns :the journal of the american dental association 2011 ;142 (11) : 1269-1274.
Felton D, Cooper L, Duqum I, Minsley G, Guckes A, Haug S, Meredith P, Solie C, Avery D, Chandler ND, American College of Prosthodontists, Academy of General Dentistry,American Dental Association Council on Scientific Affairs, American Dental Hygienists’Association, National Association of Dental Laboratories, GlaxoSmithKline Consumer Healthcare. Evidence-based guidelines for the care and maintenance of complete dentures:a publication of the American College of Prosthodontists. J Am Dent Assoc. 2011; 142Suppl 1:1S-20S
Kim E, Driscoll CF, Minah GE. The effect of a denture adhesive on the colonization of Candida species in vivo. J Prosthodont. 2003; 12(3):187-91
Sato Y, Kaiba Y, Hayakawa I. Evaluation of den- ture retention and ease of removal from oral mucosa on a new gel-type denture adhesive. NihonHotetsuShikaGakkaiZasshi. 2008;52(2): 175-182.
Pradies G, Sanz I, Evans O, Martnez F, Sanz M. Clinical study comparing the efcacy of two denture adhesives in complete denture patients. Int J Prosthodont 2009;22(4):361-367.
Kulak Y, Ozcan M, Arikan A. Subjective assess- ment by patients of the efciency of two den- ture adhesive pastes. J Prosthodont 2005;14(4): 248-252.
A Yadav, S Yadav Denture adhesives- their stand in prosthodontics The Journal of Indian prosthodontic society 2005 ; 5(2) :62-64
Dr. Hemamalini Balaji , Dr. Dhanraj Denture adhesives- A review International journal of science and research 2017; 6 (4):1156
Vuong C, Otto M. Staphylococcus epidermidis infections. Journal Microbes and Infection. 2002; 4:481–489.
Vollmer W, Blanot D, de Pedro MA. ;Peptidoglycan structure and architecture. FEMS Microbiol. Rev. 2008 ;32: 149–167.
Jeffrey M. Skerker and Howard C. Berg :Direct observation of extension and retraction of type IV pili Proc Natl Acad Sci U S A. 2001; 98(12): 6901–6904.
Stafford GD, Russell C: Efficiency of denture adhesives and their possible influence on oral microorganisms . J Dent Res 1971;50: 832-6.
-Bartels HA: Bacteriological appraisal of adhesive denture powders .L Dent Res 1945;24:15-6
Ekstrand K, Hensten-Pettersen A, Kullmann A. Denture adhesives: Cytotoxicity, microbial contamination, and formaldehyde content.J Prosthet Dent. 1993; 69(3):314-317
Benedita sampaio -Maia,Maria Helena,Figuerial,Patricia Sousa-Rodrigues, Maria Helena Fernandes, Crispian Scully.The effect of denture adhesives on candida albicans growth in vitro. Gerodontology 2012; 29(2):e348-e356.
Stecher P.G., Windholz M., Leahy D.S.,Bolton D.M., and Eaton L .G.The Merck Index.8Th Ed.Rahway, NJ. Merck and Co.Inc.1968.
Shay K, Cited from Bouchers prosthodontic treatment for edentulous patient b. 11th Ed.ST. Louis Mosby – Year Book Inc.1997.
Reither W. Effect of adhesive powders on the fit and position of complete dentures . Dent. Abst. 1957; 2:154-5.
Dr Shilpa Kalra, Dr Tulika Tripathi , Dr Priyank Rai: infection control in orthodontics Journal of orthodontics and endodontics 2015 ; 1 :1-12
DOI: http://dx.doi.org/10.26477/idj.v39i2.125
Refbacks
- There are currently no refbacks.
--------------------------------------------------------------------------------------------
ISSN (Print): 2307-4779 | ISSN (Online): 2411-9741