Effect of Thyme Water Extract on Commonly Found Oral and Root Canal Bacteria (A Comparative Study)
Abstract
Background and objectives: Bacteria and their products play an essential role in the pathogenesis of pulpo-periapical disease. The purpose of this study was to compare the anti-microbial activity of thyme water extract on selected bacterial isolates and culti- vated root canal swabs in comparison with different concentrations of sodium hypochlorite (NaOCl).
Materials and Methods: Clinical isolates of Staphylococcus aureus, Moraxella catarrhalis, and Klebsiella pneumoniae were selected. Microbiological samples from infected root canals were taken from forty five male and female patients in age group of 15-45 years old during the first visit of root canal therapy. Antibacterial assay were done by agar well diffusion method and microdilution assay.
Results: The best antimicrobial activity for thyme extract was noticed at Moraxella catarrhalis and failed to show any significant effect on Klebsiella pneumoniae. The results revealed that 2% sodium hypochlorite are more effective than thyme water extract, on evaluated bacterial isolates and swabs.
Conclusion: From bacteriological point of view, it could be demonstrated that water extracts of thyme (infusion method), may be useful for root canal irrigation with their antibacterial effect.
Keywords
Full Text:
PDFReferences
Kovac J, Kovac D. Effect of irrigation solutions in en- dodontic therapy. A systematic review. Bratisl Lek Listy. 2011;112:410-5.
Grossman LI, Oliet S, Carlos DR. Endodontic practice. 11th ed. Philadelphia; Lea and Febiger. 1991;179-227.
ByströmA,HapponenR-P,etal.Healingofperiapicallesionsof pulpless teeth after endodontic treatment with controlled asep- sis. Endod Dent Traumatol. 1987;3:58-63.
Sjögren U, Figdor D, et al. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J. 1997;30:297-306.
Fedorowicz Z, Nasser M, Sequeira-Byron P, de Souza RF, Carter B, Heft M. Irrigants for non-surgical root canal treat- ment in mature permanent teeth. Cochrane Database Syst Rev. 2012.
Bergenholtz G. Inflammatory responses of the dental pulp to bacterial irritation. J Endodont. 1981;7:100-4.
Sundqvist G. Taxonomy, ecology, and pathogenicity of
the root canal flora. Oral Surg Oral Med Oral Pathol.
;78:522-30.
Baumgartner JC, Falkler WA. Bacteria in the apical 5mm of
infected root canals. J Endod. 1991;17:380-3.
Barbosa C, Goncalves R, Siqueira J, De Uzeda M. Evalua- tion of the antibacterial activities of calcium hydroxide, chlo- rohexidine and comphorated parachlocophenol as intracanal medication. A clinical and laboratory study. J. Endodon.
;23:297-300.
YamaguchiM,YoshidaK,SuzukiR,NakamuraH.Rootcanal
irrigation with citric acid solution. J. Endod. 1996;22:27-9. 11. LožienK,ŠipailienA,MaždžierienR,LabokasJ,Rimantas PV. Composition of Essential Oil, Radical Scavenging and Antibacterial Properties of Interspecific Hybrid Thymus ×
oblongifolius Opiz. Rec. Nat. Prod. 2012;6:84-8.
Pourmortazavi S.M. Hajimirsadeghi S.S. Supercritical flu- id extraction in plant essential and volatile oil analysis. J
Chromatogr. 2007;1163:20-4.
Babovic N, Djilas S, Jadranin M, Vajs V, Ivanovic J, Petro-
vic S, Zizovic I. Supercritical carbon dioxide extraction of antioxidant fractions from selected Lamiaceae herbs and their antioxidant capacity. Innov Food Sci Emerg Technol. 2010;11:98-107.
Petersson L, Edwardsson S, Arends J. Antimicrobial ef- fect of a dental varnish, in vitro. Swedish dental journal. 1992;16:183-9.
Solano E, Castillejos Cruz C, Alvarez Estrada L, Arellano Villavicencio AVM, De la Luz Lopez-Martinez M, Rios- Gomez R. Effect of the essential oil, infusion and ethanol extract of Thymus vulgar L., on the growth in vitro of group A beta-hemolytic Streptococcus pyogenes. TIP Revista Es- pecializada en Ciencias Quimico-Biologicas. 2006;9:73-7.
Goyal P, Khanna A, Chauhan A, Chauhan G, Kaushik P. In vitro evaluation of crude extracts of Catharanthus ro- seus for potential antibacterial activity. Int J Green Pharm. 2008;2:176-81.
Levinson W.Review of medical microbiology and immu- nology. McCraw-Hill. New York. 2004.
YogishKV,MaheepBandKanikaS.Invitroevaluationof antibacterial activity of an herbal dentifrice against Strepto- coccus mutans and Lactobacillus acidophilus. Indian J Dent Res. 2008;19(1).
Sushma Jaju, Prashant P. Jaju. Newer root canal irrigants in horizon: A review. International Journal of Dentistry. 2011;1-9. 20. Bahena CA, García SS, Morales CT, González-Rodríguez VM, Baires-Várguez L. Use of sodium hypochlorite in root canal irrigation. Opinion survey and concentration in commercial products. Revista Odontológica Mexicana.
;16:252-58.
Ouwehand AC, Tiihonen K, Kettunen H, Peuranen S,
Schulze H, Rautonen N. In vitro effects of essential oils on potential pathogens and beneficial members of the normal microbiota. Veterinarni Medicina. 2010;55:71-8.
Ghazanfar S. Handbook of Arabian medicinal plants. CRC press. 1994; Roca Raton.
WaltonRE,TorabinejadM.PrinciplesandPracticeofEndo- dontics. Philadelphia: Saunders, 1989.
DOI: http://dx.doi.org/10.26477/idj.v35i1.153
Refbacks
- There are currently no refbacks.
--------------------------------------------------------------------------------------------
ISSN (Print): 2307-4779 | ISSN (Online): 2411-9741